Surfing on a new wave of single-molecule fluorescence methods.

Journal article

Single-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule toolbox. In this review, we discuss recent advances in fluorescence instrumentation and assays: these methods are characterized by a substantial increase in complexity of the instrumentation or biological samples involved. Specifically, we describe new multi-laser and multi-colour fluorescence spectroscopy and imaging techniques, super-resolution microscopy imaging and the development of instruments that combine fluorescence detection with other single-molecule methods such as force spectroscopy. We also highlight two pivotal developments in basic and applied biosciences: the new information available from detection of single molecules in single biological cells and exciting developments in fluorescence-based single-molecule DNA sequencing.

Authors: Hohlbein, J; Gryte, K; Heilemann, M; Kapanidis, A

• Publication status: Published
• Journal: Physical biology
• Volume: 7
• Issue: 3
• Pages: 031001
• Publication date: 2010-01-01
• DOI: 10.1088/1478-3975/7/3/031001
• EISSN: 1478-3975
• ISSN: 1478-3967
• Language: English
• Keywords: Microscopy, Fluorescence; Surface Properties; Fluorescence; Sequence Analysis, DNA