Suppression of insulin release by galanin and somatostatin is mediated by a G-protein. An effect involving repolarization and reduction in cytoplasmic free Ca2+ concentration.

Journal article

The effects of galanin and somatostatin on insulin release, membrane potential, and cytoplasmic free Ca2+ concentration [( Ca2+]i) were investigated using beta-cells isolated from obese hyperglycemic mice. Whereas insulin release was measured in a column perifusion system, membrane potential and [Ca2+]i were measured with the fluorescent indicators bisoxonol (bis-(1,3-diethylthiobarbiturate)trimethineoxonol) and quin 2, in cell suspensions in a cuvette. Galanin (16 nM) and somatostatin (400 n...

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Authors: Nilsson, T; Arkhammar, P; Rorsman, P; Berggren, P

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 264
• Issue: 2
• Pages: 973-980
• Publication date: 1989-01-01
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Somatostatin; Kinetics; Mice, Obese; Neuropeptides; Membrane Potentials; Potassium; Calcium; Islets of Langerhans; Cell Membrane; Gallopamil; Male; Insulin; Glucose; Peptides; Hyperglycemia; Animals; Galanin; Mice; GTP-Binding Proteins; Female