Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae

Journal article

Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filament-forming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frame-GFP collection in Saccharomyces cerevisiae. We discovered 9 novel filament-forming proteins and also confirmed those identified previously. Out of the 4159 strains, we found 23 proteins, mostly metabolic enzymes, that are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery, glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases (Ura7p and Ura8p) and two asparagine synthetases (Asn1p and Asn2p) form filaments both in the cytoplasm and in the nucleus. Live imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in Saccharomyces cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated.

Authors: Liu, J; Shen, Q; Kassim, H; Huang, Y; Li, H

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Elsevier
• Journal: Journal of Genetics and Genomics
• Volume: 43
• Issue: 6
• Pages: 393–404
• Publication date: 2016-04-01
• Acceptance date: 2016-03-28
• DOI: 10.1016/j.jgg.2016.03.008
• ISSN: 1673-8527
• Keywords: glycolysis; cytoophidium; CTP synthase; 40 intracellular compartmentation; Saccharomyces cerevisiae; glutamine; metabolic enzyme