The subclonal complexity of STIL-TAL1+ T-cell acute lymphoblastic leukaemia

Journal article

Single-cell genetics were used to interrogate clonal complexity and the sequence of mutational events in STIL-TAL1+ T-ALL. Single-cell multicolour FISH was used to demonstrate that the earliest detectable leukaemia subclone contained the STIL-TAL1 fusion and copy number loss of 9p21.3 (CDKN2A/CDKN2B locus), with other copy number alterations including loss of PTEN occurring as secondary subclonal events. In three cases, multiplex qPCR and phylogenetic analysis were used to produce branching e...

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Authors: Furness, CL; Braga Mansur, M; Weston, VJ; Ermini, L; van Delft, FW

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Springer Nature
• Journal: Leukemia
• Volume: 32
• Issue: 9
• Pages: 1984-1993
• Place of publication: England
• Publication date: 2018-03-20
• Acceptance date: 2017-12-18
• DOI: 10.1038/s41375-018-0046-8
• EISSN: 1476-5551
• ISSN: 0887-6924
• Pmid: 29556024
• Language: English
• Keywords: multiplex polymerase chain Reaction; genome-wide association study; FFR; cell line, tumor; heterografts; disease models, animal; clonal evolution; humans; infant; young adult; oncogene proteins, fusion; mutation; adult; precursor t-cell lymphoblastic Leukemia-Lymphoma; intracellular signaling peptides and proteins; child; polymorphism, single nucleotide; child, preschool; single-cell analysis; In Situ Hybridization, Fluorescence; PTEN Phosphohydrolase; animals; alleles; adolescent; t-cell acute lymphocytic leukemia Protein 1