- Abstract:
-
Single-molecule fluorescence and Förster resonance energy transfer (smFRET) are important tools for studying molecular heterogeneity, cellular organization, and protein structure in living cells. However, in vivo smFRET studies are still very challenging, and a standardized approach for robust in vivo smFRET measurements is still missing. Here, we synthesized protected DNAs with chemically linked ends as robust in vivo nano-rulers. We efficiently internalized doubly-labeled end-sealed DNA sta...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 1.3MB)
-
- Publisher copy:
- 10.1039/C6SC00639F
-
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- Publisher:
- Royal Society of Chemistry Publisher's website
- Journal:
- Chem. Sci. Journal website
- Volume:
- 7
- Issue:
- 7
- Pages:
- 4418-4422
- Publication date:
- 2016-03-23
- DOI:
- EISSN:
-
2041-6539
- ISSN:
-
2041-6520
- Pubs id:
-
pubs:634477
- URN:
-
uri:f015d281-e65a-44a3-8f7d-18ed8f30e4ba
- UUID:
-
uuid:f015d281-e65a-44a3-8f7d-18ed8f30e4ba
- Local pid:
- pubs:634477
- Paper number:
- 7
- Keywords:
- Copyright holder:
- Plochowietz et al.
- Copyright date:
- 2016
- Notes:
- © the Author(s). This journal is © The Royal Society of Chemistry 2016.
Journal article
Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence
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Engineering and Physical Sciences Research Council
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European Research Council
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Biotechnology and Biological Sciences Research Council
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German National Academic Foundation
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