Journal article icon

Journal article

Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence

Abstract:

Single-molecule fluorescence and Förster resonance energy transfer (smFRET) are important tools for studying molecular heterogeneity, cellular organization, and protein structure in living cells. However, in vivo smFRET studies are still very challenging, and a standardized approach for robust in vivo smFRET measurements is still missing. Here, we synthesized protected DNAs with chemically linked ends as robust in vivo nano-rulers. We efficiently internalized doubly-labeled end-sealed DNA sta...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

Actions


Access Document


Files:
Publisher copy:
10.1039/C6SC00639F

Authors


More by this author
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Role:
Author
Engineering and Physical Sciences Research Council More from this funder
European Research Council More from this funder
Biotechnology and Biological Sciences Research Council More from this funder
German National Academic Foundation More from this funder
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chem. Sci. Journal website
Volume:
7
Issue:
7
Pages:
4418-4422
Publication date:
2016-03-23
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Pubs id:
pubs:634477
URN:
uri:f015d281-e65a-44a3-8f7d-18ed8f30e4ba
UUID:
uuid:f015d281-e65a-44a3-8f7d-18ed8f30e4ba
Local pid:
pubs:634477
Paper number:
7
Keywords:

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP