Journal article
Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence
- Abstract:
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Single-molecule fluorescence and Förster resonance energy transfer (smFRET) are important tools for studying molecular heterogeneity, cellular organization, and protein structure in living cells. However, in vivo smFRET studies are still very challenging, and a standardized approach for robust in vivo smFRET measurements is still missing. Here, we synthesized protected DNAs with chemically linked ends as robust in vivo nano-rulers. We efficiently internalized doubly-labeled end-sealed DNA sta...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 1.3MB)
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- Publisher copy:
- 10.1039/C6SC00639F
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Authors
Funding
Engineering and Physical Sciences Research Council
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European Research Council
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Biotechnology and Biological Sciences Research Council
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German National Academic Foundation
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Bibliographic Details
- Publisher:
- Royal Society of Chemistry Publisher's website
- Journal:
- Chem. Sci. Journal website
- Volume:
- 7
- Issue:
- 7
- Pages:
- 4418-4422
- Publication date:
- 2016-03-23
- Acceptance date:
- 2016-03-21
- DOI:
- EISSN:
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2041-6539
- ISSN:
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2041-6520
- Source identifiers:
-
8795
Item Description
- Keywords:
- Pubs id:
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pubs:634477
- UUID:
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uuid:f015d281-e65a-44a3-8f7d-18ed8f30e4ba
- Local pid:
- pubs:634477
- Deposit date:
- 2017-02-13
Terms of use
- Copyright holder:
- Plochowietz et al
- Copyright date:
- 2016
- Notes:
- © the Author(s). This journal is © The Royal Society of Chemistry 2016.
- Licence:
- CC Attribution (CC BY)
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