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Journal article

Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence

Abstract:

Single-molecule fluorescence and Förster resonance energy transfer (smFRET) are important tools for studying molecular heterogeneity, cellular organization, and protein structure in living cells. However, in vivo smFRET studies are still very challenging, and a standardized approach for robust in vivo smFRET measurements is still missing. Here, we synthesized protected DNAs with chemically linked ends as robust in vivo nano-rulers. We efficiently internalized doubly-labeled end-sealed DNA sta...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1039/C6SC00639F

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Engineering and Physical Sciences Research Council More from this funder
European Research Council More from this funder
Biotechnology and Biological Sciences Research Council More from this funder
German National Academic Foundation More from this funder
Publisher:
Royal Society of Chemistry Publisher's website
Journal:
Chem. Sci. Journal website
Volume:
7
Issue:
7
Pages:
4418-4422
Publication date:
2016-03-23
Acceptance date:
2016-03-21
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Source identifiers:
8795
Keywords:
Pubs id:
pubs:634477
UUID:
uuid:f015d281-e65a-44a3-8f7d-18ed8f30e4ba
Local pid:
pubs:634477
Deposit date:
2017-02-13

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