Selective abolishment of pyrimidine nucleoside kinase activity of herpes simplex virus type 1 thymidine kinase by mutation of alanine-167 to tyrosine.

Journal article

Herpes simplex virus type 1 (HSV-1) encodes a thymidine kinase (TK) that markedly differs from mammalian nucleoside kinases in terms of substrate specificity. It recognizes both pyrimidine 2'-deoxynucleosides and a variety of purine nucleoside analogs. Based on a computer modeling study and in an attempt to modify this specificity, an HSV-1 TK mutant enzyme containing an alanine-to-tyrosine mutation at amino acid position 167 was constructed. Compared with wild-type HSV-1 TK, the purified mut...

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Authors: Degrève, B; Esnouf, R; De Clercq, E; Balzarini, J

• Publication status: Published
• Journal: Molecular pharmacology
• Volume: 58
• Issue: 6
• Pages: 1326-1332
• Publication date: 2000-12-01
• EISSN: 1521-0111
• ISSN: 0026-895X
• Language: English
• Keywords: Bone Neoplasms; Antiviral Agents; Point Mutation; Alanine; Humans; Transduction, Genetic; Tyrosine; Amino Acid Substitution; Cell Division; Herpesvirus 1, Human; Ganciclovir; Tumor Cells, Cultured; Osteosarcoma; Purine Nucleosides; Thymidine Kinase; Phosphorylation; Pyrimidine Nucleosides