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Selective abolishment of pyrimidine nucleoside kinase activity of herpes simplex virus type 1 thymidine kinase by mutation of alanine-167 to tyrosine.

Abstract:

Herpes simplex virus type 1 (HSV-1) encodes a thymidine kinase (TK) that markedly differs from mammalian nucleoside kinases in terms of substrate specificity. It recognizes both pyrimidine 2'-deoxynucleosides and a variety of purine nucleoside analogs. Based on a computer modeling study and in an attempt to modify this specificity, an HSV-1 TK mutant enzyme containing an alanine-to-tyrosine mutation at amino acid position 167 was constructed. Compared with wild-type HSV-1 TK, the purified mut...

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Publication status:
Published

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Journal:
Molecular pharmacology
Volume:
58
Issue:
6
Pages:
1326-1332
Publication date:
2000-12-05
EISSN:
1521-0111
ISSN:
0026-895X
URN:
uuid:efe1cebe-ccc6-4845-8fb3-884bb5dc89f6
Source identifiers:
17265
Local pid:
pubs:17265

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