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Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit.

Abstract:

We review recent results on the complete structure of the archaeal RNAP (RNA polymerase) enzyme of Sulfolobus shibatae. We compare the three crystal forms in which this RNAP packs (space groups P2₁2₁2₁, P2₁2₁2 and P2₁) and provide a preliminary biophysical characterization of the newly identified 13-subunit Rpo13. The availability of different crystal forms for this RNAP allows the analysis of the packing degeneracy and the intermolecular interactions that determine this degeneracy. We observ...

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Publication status:
Published

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Publisher copy:
10.1042/bst0390025

Authors


Peralta, B More by this author
Ondiviela, M More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
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Journal:
Biochemical Society transactions
Volume:
39
Issue:
1
Pages:
25-30
Publication date:
2011-01-05
DOI:
EISSN:
1470-8752
ISSN:
0300-5127
URN:
uuid:efa290ed-cff6-435b-9ad7-c6306ccaecf5
Source identifiers:
124500
Local pid:
pubs:124500

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