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Highly sensitive direct detection and quantification of Burkholderia pseudomallei bacteria in environmental soil samples by using real-time PCR.

Abstract:

The soil bacterium and potential biothreat agent Burkholderia pseudomallei causes the infectious disease melioidosis, which is naturally acquired through environmental contact with the bacterium. Environmental detection of B. pseudomallei represents the basis for the development of a geographical risk map for humans and livestock. The aim of the present study was to develop a highly sensitive, culture-independent, DNA-based method that allows direct quantification of B. pseudomallei from soil...

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Publisher copy:
10.1128/aem.00735-11

Authors


Göhler, A More by this author
Topfstedt, E More by this author
Wuthiekanun, V More by this author
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Journal:
Applied and environmental microbiology
Volume:
77
Issue:
18
Pages:
6486-6494
Publication date:
2011-09-05
DOI:
EISSN:
1098-5336
ISSN:
0099-2240
URN:
uuid:ef1d79ab-aee9-4162-8e0d-0840bc51ca06
Source identifiers:
167569
Local pid:
pubs:167569

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