Scanning electron microscopy of cells and tissues under fully hydrated conditions.

Journal article

A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is approximately 100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogol...

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Authors: Thiberge, S; Nechushtan, A; Sprinzak, D; Gileadi, O; Behar, V

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 101
• Issue: 10
• Pages: 3346-3351
• Publication date: 2004-03-01
• DOI: 10.1073/pnas.0400088101
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Staining and Labeling; Cytological Techniques; Water; CHO Cells; Histological Techniques; Cricetinae; Gold; Hela Cells; Mice; Electrons; Trypanosoma brucei brucei; Microscopy, Electron, Scanning; Humans; Animals; Scattering, Radiation