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Scanning electron microscopy of cells and tissues under fully hydrated conditions.

Abstract:

A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is approximately 100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogol...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.0400088101

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Genomics Consortium
Role:
Author
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Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
101
Issue:
10
Pages:
3346-3351
Publication date:
2004-03-05
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
URN:
uuid:ef04b566-f3a9-4832-ba64-43c3bebe795c
Source identifiers:
32637
Local pid:
pubs:32637

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