Derivation and characterisation of the human embryonic stem cell line, OxF1.

Journal article

OxF1 is a human embryonic stem cell line derived from a surplus embryo donated through the Oxford IVF clinic. The cells have a stable 46 XX karyotype and show expression of Oct 4, Nanog and TRA-1-60. Embryoid bodies differentiate into cells that represent all three germ layers as demonstrated by immunohistochemical localisation of beta III tubulin, nestin, desmin, smooth muscle actin, Gata 6 and cytokeratin 18. Directed differentiation through haematopoiesis has been demonstrated.

Authors: Brook, F; Cowley, S; Evans, E; Turner, K; James, W

• Publication status: Published
• Journal: In vitro cellular and developmental biology. Animal
• Volume: 46
• Issue: 3-4
• Pages: 173-177
• Publication date: 2010-04-01
• DOI: 10.1007/s11626-010-9283-5
• EISSN: 1543-706X
• ISSN: 1071-2690
• Language: English
• Keywords: Cell Lineage; Humans; Embryonic Stem Cells; Cell Differentiation; Cell Culture Techniques; Germ Layers; Cell Line; Great Britain; Embryo, Mammalian; Myeloid Cells