Journal article
A tissue-like platform for studying engineered quiescent human T-cells' interactions with dendritic cells
- Abstract:
- Research in the field of human immunology is restricted by the lack of a system that reconstitutes the in-situ activation dynamics of quiescent human antigen-specific T-cells interacting with dendritic cells. Here we report a tissue-like system that recapitulates the dynamics of engineered primary human immune cell. Our approach facilitates real-time single cell manipulations, tracking of interactions and functional responses complemented by population-based measurements of cytokines, activation status and proliferation. As a proof of concept, we recapitulate immunological phenomenon such as CD4 help to CD8 T-cells through enhanced maturation of DCs and effect of PD-1 checkpoint blockades. In addition, we characterise unique dynamics of T-cell/DC interactions as a function of antigen affinity.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 3.1MB, Terms of use)
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- Publisher copy:
- 10.7554/elife.48221
Authors
- Publisher:
- eLife Sciences Publications
- Journal:
- eLife More from this journal
- Volume:
- 8
- Article number:
- e48221
- Publication date:
- 2019-09-25
- Acceptance date:
- 2019-09-24
- DOI:
- EISSN:
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2050-084X
- Pmid:
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31552826
- Language:
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English
- Keywords:
- Pubs id:
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pubs:1059094
- UUID:
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uuid:eea5f864-fcca-42cd-bd7f-9fbad3d1cc06
- Local pid:
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pubs:1059094
- Source identifiers:
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1059094
- Deposit date:
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2019-10-24
Terms of use
- Copyright holder:
- Abu Shah et al
- Copyright date:
- 2019
- Notes:
- © 2019, Abu Shah et al. This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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