- Abstract:
-
During the construction of yeast artificial chromosome (YAC) libraries to facilitate mapping of the human genome, two YACs may be cotransformed into the same yeast cell, making further analysis very difficult. We present a simple method to rescue the required YAC that utilizes the segregation of chromosomes at meiosis. In brief, we crossed the cotransformed yeast cell with a non-YAC-containing strain and induced the resulting diploid to sporulate and undergo meiosis. The new haploid generatio...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/1050-3862(93)90007-6
-
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- Journal:
- Genetic analysis, techniques and applications
- Volume:
- 10
- Issue:
- 6
- Pages:
- 123-127
- Publication date:
- 1993
- DOI:
- ISSN:
-
1050-3862
- URN:
-
uuid:ee6683e8-036c-4c85-b882-5b3723578ff1
- Source identifiers:
-
438083
- Local pid:
- pubs:438083
- Copyright date:
- 1993
Journal article
Rescue of a single yeast artificial chromosome from a cotransformation event utilizing segregation at meiosis.
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