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Rescue of a single yeast artificial chromosome from a cotransformation event utilizing segregation at meiosis.

Abstract:

During the construction of yeast artificial chromosome (YAC) libraries to facilitate mapping of the human genome, two YACs may be cotransformed into the same yeast cell, making further analysis very difficult. We present a simple method to rescue the required YAC that utilizes the segregation of chromosomes at meiosis. In brief, we crossed the cotransformed yeast cell with a non-YAC-containing strain and induced the resulting diploid to sporulate and undergo meiosis. The new haploid generatio...

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Publication status:
Published

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Authors


Németh, AH More by this author
Coleman, MP More by this author
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Journal:
Genetic analysis, techniques and applications
Volume:
10
Issue:
6
Pages:
123-127
Publication date:
1993
DOI:
ISSN:
1050-3862
URN:
uuid:ee6683e8-036c-4c85-b882-5b3723578ff1
Source identifiers:
438083
Local pid:
pubs:438083

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