Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.

Journal article

A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli.

Authors: El-Sagheer, A; Sanzone, A; Gao, R; Tavassoli, A; Brown, T

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 108
• Issue: 28
• Pages: 11338-11343
• Publication date: 2011-07-01
• DOI: 10.1073/pnas.1101519108
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Sequence Homology, Nucleic Acid; DNA-Directed DNA Polymerase; Base Sequence; Molecular Mimicry; Triazoles; Escherichia coli; Molecular Structure; Molecular Sequence Data; DNA; Oligodeoxyribonucleotides; Polymerase Chain Reaction