In vitro synthesis of transfer RNA. II. Identification of required enzymatic activities.

Journal article

We have shown that the synthesis of active su+III tRNATyr from a phi80psu+III DNA template requires the action of four distinct enzymatic activities. The first of these, DNA-dependent RNA polymerase, catalyzes the formation of a large molecular weight transcript, initiating synthesis at a specific site 41 nucleotides proximal to the 5' end of the su+III tRNATyr structural gene and continuing at least 100 nucleotides beyond the 3' terminus of the su+III tRNATyr sequence. The second required co...

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Authors: Bikoff, E; LaRue, B; Gefter, M

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 250
• Issue: 16
• Pages: 6248-6255
• Publication date: 1975-08-01
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: RNA, Viral; RNA, Bacterial; Ribonucleases; Transcription, Genetic; Tyrosine; Coliphages; RNA, Transfer; Uracil Nucleotides; DNA; DNA-Directed RNA Polymerases; Escherichia coli; RNA Nucleotidyltransferases