Effects of monoclonal antibodies to the alpha and beta chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions.

Dongworth, D; Gotch, F; Hildreth, J; Morris, A; Mcmichael, A

Journal article

Effects of monoclonal antibodies to the alpha and beta chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions.

Abstract:: The ability of two antibodies, one specific for the alpha chain, p180, and the other for the beta chain, p95, of the human lymphocyte function-associated (LFA-1) antigens, to inhibit T cell function was measured. Both antibodies inhibited T cell-mediated lysis of virus-infected target cells and of K562 cells. Only the anti-beta chain antibody inhibited natural killer cell lysis of K562. The antibodies inhibited cytotoxic T lymphocyte cell (CTL) lysis of HLA-mismatched target cells in the presence of concanavalin A at 6.25-12.5 micrograms/ml, but at higher doses of Con A no inhibition was seen. When the lytic process was divided into calcium-independent (adherence) and -dependent (lysis) steps the antibodies were found to block at the initial step of conjugate formation. The effects of these antibodies on T cell proliferative responses showed that responses to antigens, alloantigens, mitogens and anti-CD3 (UCHT1) antibody were greatly inhibited. All of these responses are adherent cell dependent and proliferation of adherent cell-depleted mononuclear cells to Sepharose-coupled UCHT1 was not inhibited by anti-LFA-1 antibodies. Proliferation to paired anti-CD2 (T11) antibodies was also only weakly inhibited. Release of interferon-gamma by CTL on contact with target cells was also inhibited by anti-LFA antibody. These results are evidence that the LFA antigen is necessary for a nonspecific interaction with antigen-presenting cells that is essential for activation of T cells through the CD3-T cell receptor complex.

Publication status:: Published

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APA Style

Dongworth, D., Gotch, F., Hildreth, J., Morris, A., & Mcmichael, A. (1985). Effects of monoclonal antibodies to the alpha and beta chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions. European Journal of Immunology, 15(9), 888–892.

MLA Style

Dongworth, D, et al. “Effects of Monoclonal Antibodies to the Alpha and Beta Chains of the Human Lymphocyte Function-Associated (H-LFA-1) Antigen on T Lymphocyte Functions.” European Journal of Immunology, vol. 15, no. 9, 1985, pp. 888–92.

Chicago Style

Dongworth, D, F Gotch, J Hildreth, A Morris, and A Mcmichael. 1985. “Effects of Monoclonal Antibodies to the Alpha and Beta Chains of the Human Lymphocyte Function-Associated (H-LFA-1) Antigen on T Lymphocyte Functions.” European Journal of Immunology 15 (9): 888–92.
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