Chromosome 2p, 3p, 5q and 18q status in sporadic gastric cancer.

Journal article

AIM: The genetic make-up of gastric cancers in low-risk population groups from South Africa is largely unknown. The purpose of this study was to ascertain the incidence of microsatellite instability and loss of heterozygosity in this population. METHODS: Thirty-seven gastrectomy specimens for sporadic gastric cancer were analysed for the following clinicopathological parameters: age, gender, race, histopathological type, size of tumour, lymph node status and the presence/absence of Helicobacter pylori. DNA was then extracted from paraffin-embedded tissue and seven microsatellite markers in 2p, 3p, 5q and 18q loci were examined using automated DNA fluorescent technology. RESULTS: Only eight cases showed microsatellite instability (MSI) for one marker and were thus categorised as MSI-low. In the 3p region, loss of heterozygosity (LOH) was detected in 21.7-38.3% of informative cases, whilst in the 18q region CLOH ranged from 25 to 38.4%. LOH was not seen in the 2p locus and only one case showed LOH in the 5q region. When the molecular changes were compared with clinicopathological parameters, a statistically significant relationship did not emerge with any single parameter. CONCLUSIONS: This study shows that sporadic gastric cancer from a low-risk population in South Africa is MSI-low or MSI-stable, and that LOH in the 3p and 18q regions is similar to that seen in other low-risk populations from different geographical regions.

Authors: Chetty, R; Naidoo, R; Tarin, M; Sitti, C

• Publication status: Published
• Journal: Pathology
• Volume: 34
• Issue: 3
• Pages: 275-281
• Publication date: 2002-06-01
• DOI: 10.1080/00313020220131354
• EISSN: 1465-3931
• ISSN: 0031-3025
• Language: English
• Keywords: Genes, APC; Aged, 80 and over; Carrier Proteins; Middle Aged; Neoplasm Proteins; Female; Adaptor Proteins, Signal Transducing; Loss of Heterozygosity; Adult; Genes, DCC; Chromosomes, Human, 1-3; South Africa; DNA-Binding Proteins; Nuclear Proteins; African Continental Ancestry Group; Chromosomes, Human, Pair 18; Microsatellite Repeats; Proto-Oncogene Proteins; Male; Stomach Neoplasms; MutS Homolog 2 Protein; India; European Continental Ancestry Group; DNA, Neoplasm; Aged; Humans; Chromosomes, Human, Pair 5; Polymerase Chain Reaction