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A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes

Abstract:

Background. The emergence and co-circulation of two different clades (clade 1 and 2) of H5N1 influenza viruses in Vietnam necessitates the availability of a diagnostic assay that can detect both variants. Results. We developed a single real-time RT-PCR assay for detection of both clades of H5N1 viruses, directly from clinical specimens, using locked nucleic acid TaqMan probes. Primers and probe used in this assay were designed based on a highly conserved region in the HA gene of H5N1 viruses....

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Publisher copy:
10.1186/1743-422X-7-46

Authors


Tran Tan, T More by this author
Pawestri, HA More by this author
Syahrial, H More by this author
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Journal:
Virology Journal
Volume:
7
Publication date:
2010
DOI:
EISSN:
1743-422X
URN:
uuid:ededb2c0-3934-477f-a96a-05e6af5a83a9
Source identifiers:
92786
Local pid:
pubs:92786
Language:
English

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