The linear assembly of a pure glycoenzyme.
- A labor of love: The synthesis of an active pure enzyme (RNase) glycoform by the native chemical ligation of a (glyco)peptide and peptide thioester fragments required numerous painstaking steps: the strategy was optimized, active inteins accessed, redox conditions fine-tuned, and glycoamino acid building blocks synthesized.
- Publication status:
- Publisher copy:
- Copyright date: