The linear assembly of a pure glycoenzyme.

Journal article

A labor of love: The synthesis of an active pure enzyme (RNase) glycoform by the native chemical ligation of a (glyco)peptide and peptide thioester fragments required numerous painstaking steps: the strategy was optimized, active inteins accessed, redox conditions fine-tuned, and glycoamino acid building blocks synthesized.

Authors: Davis, B

• Publication status: Published
• Journal: Angewandte Chemie (International ed. in English)
• Volume: 48
• Issue: 26
• Pages: 4674-4678
• Publication date: 2009-01-01
• DOI: 10.1002/anie.200806246
• EISSN: 1521-3773
• ISSN: 1433-7851
• Language: English
• Keywords: Glycoproteins; Glycopeptides; Oxidation-Reduction; Ribonucleases; Protein Folding; Inteins; Protein Engineering