Molecular epidemiology of Plasmodium falciparum resistance to antimalarial drugs in Indonesia.

Journal article

The extent of gene polymorphisms associated with resistance to chloroquine and sulfadoxine-pyrimethamine was examined in field isolates of Plasmodium falciparum from Indonesia. Eight malaria-endemic areas, representing a broad region of the western and eastern Indonesian Archipelago were surveyed. Blood from 20-50 patients was collected at each site, DNA was isolated, and the sequences of four different genes (dihydrofolate reductase [dhfr], dihydropteroate synthase [dhps], P. falciparum multidrug resistance 1 [pfmdr1], and P. falciparum chloroquine resistance transporter [pfcrt]) were analyzed using polymerase chain reaction and restriction fragment length polymorphisms to detect polymorphisms previously shown to be associated with resistance. This analysis identified polymorphisms in dhfr at 108-Asn/Thr, 16-Val, and 59-Arg. Polymorphisms in dhps were found less frequently, either 437-Gly alone or paired with 540-Glu. The pfcrt 76-Thr polymorphism was fixed in all parasite populations and pfmdr1 86-Tyr polymorphisms in all populations except in the most eastern regions. The pfmdr1 1042-Asp polymorphism occurred less frequently. These findings indicate that polymorphisms in genes associated with drug resistance in P. falciparum are found across a broad region of Indonesia.

Authors: Syafruddin, D; Asih, P; Casey, G; Maguire, J; Baird, J

• Publication status: Published
• Journal: American journal of tropical medicine and hygiene
• Volume: 72
• Issue: 2
• Pages: 174-181
• Publication date: 2005-02-01
• EISSN: 1476-1645
• ISSN: 0002-9637
• Language: English
• Keywords: DNA, Protozoan; Antimalarials; Genes, MDR; ATP-Binding Cassette Transporters; Humans; Protozoan Proteins; Drug Resistance, Multiple; Malaria, Falciparum; Polymerase Chain Reaction; Indonesia; Animals; Plasmodium falciparum