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Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine.

Abstract:

We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromodeoxyuridine. Low concentrations of EdU (1 µM) are sufficient to allow detection of incorporation in cells expressing thymidine kinase and human equilibrative nucleoside transporter 1 (hENT1). However EdU is toxic and activates the rad3-dependent checkpoint, resulting in cell cycle arrest, potentially limiting ...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gkr063

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Institution:
University of Oxford
Department:
Oxford, MPLS, Zoology
Journal:
Nucleic acids research
Volume:
39
Issue:
9
Pages:
e60
Publication date:
2011-05-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:ed9ba8ad-55b4-41a3-87c2-70f79a2550d6
Source identifiers:
209636
Local pid:
pubs:209636

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