Na+/H+ antiport and buffering capacity in human polymorphonuclear and mononuclear leucocytes.

Journal article

1. Na+/H+ antiport activity was measured in peripheral blood polymorphonuclear and mononuclear cells of 12 healthy subjects by using an intracellular pH clamp technique to determine the external Na(+)-dependent H+ efflux rate in cells loaded with a pH-sensitive fluorescent dye, bis(carboxyethyl)carboxyfluorescein. The change in external Na+ concentrations for all pH measurements was similar in both cell types. 2. A significant difference between the two types of cells was found, the polymorphonuclear leucocytes having a higher Na+/H+ antiport activity than the lymphocytes. Cellular intrinsic buffering capacity measured in the absence of HCO3- was also higher in the polymorphonuclear cells than in the lymphocytes. 3. These differences may be associated with a difference in the role of the Na+/H+ exchanger in these two types of cells, although in vivo the presence of HCO3-/Cl- exchangers may also contribute to intracellular pH homoeostasis.

Authors: Frighi, V; Ng, L; Lewis, A; Dhar, H

• Publication status: Published
• Journal: Clinical science (London, England : 1979)
• Volume: 80
• Issue: 2
• Pages: 95-99
• Publication date: 1991-02-01
• EISSN: 1470-8736
• ISSN: 0143-5221
• Language: English
• Keywords: Meglumine; Carrier Proteins; Male; Neutrophils; Hydrogen-Ion Concentration; Humans; Hydrogen; Adult; Sodium-Hydrogen Antiporter; Sodium Chloride; Amiloride; Lymphocytes; Anti-Arrhythmia Agents; Female; Leukocytes; Buffers