Structural basis for CRISPR RNA-guided DNA recognition by Cascade.

Journal article

The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids. In Escherichia coli, immunity depends on a ribonucleoprotein complex called Cascade. Here we present the composition and low-resolution structure of Cascade and show how it recognizes double-stranded DNA (dsDNA) targets in a sequence-specific manner. Cascade is a 405-kDa complex comprising five functionally essential CRISPR-asso...

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Authors: Jore, M; Lundgren, M; van Duijn, E; Bultema, J; Westra, E

• Journal: Nature structural and molecular biology
• Volume: 18
• Issue: 5
• Pages: 529-536
• Publication date: 2011-05-01
• DOI: 10.1038/nsmb.2019
• EISSN: 1545-9985
• ISSN: 1545-9993
• Language: English
• Keywords: Nucleic Acid Conformation; Escherichia coli Proteins; Binding Sites; Protein Structure, Tertiary; RNA, Bacterial; RNA, Guide; Ribonucleoproteins; Base Sequence; DNA; Escherichia coli; Structure-Activity Relationship