Journal article
Depletion of the NbCORE receptor drastically improves agroinfiltration productivity in older Nicotiana benthamiana plants
- Abstract:
- Nicotiana benthamiana is increasingly used for transient gene expression to produce antibodies, vaccines, and other pharmaceutical proteins but transient gene expression is low in fully developed, 6-8-week old plants. This low gene expression is thought to be caused by the perception of the cold shock protein (CSP) of Agrobacterium tumefaciens. The CSP receptor is contested because both NbCSPR and NbCORE have been claimed to perceive CSP. Here, we demonstrate that CSP perception is abolished in 6-week-old plants silenced for NbCORE but not NbCSPR. Importantly, older NbCORE-silenced plants support a highly increased level of GFP fluorescence and protein upon agroinfiltration. The drastic increase in transient protein production in NbCORE-depleted plants offers new opportunities for molecular farming, where older plants with larger biomass can now be used for efficient protein expression.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.6MB, Terms of use)
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- Publisher copy:
- 10.1111/pbi.14037
Authors
+ Biotechnology and Biological Sciences Research Council
More from this funder
- Funder identifier:
- https://ror.org/00cwqg982
- Grant:
- BB/R017913/1
- DTP DDT00060
+ European Research Council
More from this funder
- Funder identifier:
- https://ror.org/0472cxd90
- Grant:
- 101019324
- Publisher:
- Wiley
- Journal:
- Plant Biotechnology Journal More from this journal
- Volume:
- 21
- Issue:
- 6
- Pages:
- 1103-1105
- Place of publication:
- England
- Publication date:
- 2023-03-14
- Acceptance date:
- 2023-02-24
- DOI:
- EISSN:
-
1467-7652
- ISSN:
-
1467-7644
- Pmid:
-
36917445
- Language:
-
English
- Keywords:
- Pubs id:
-
1333347
- Local pid:
-
pubs:1333347
- Deposit date:
-
2025-04-29
- ARK identifier:
Terms of use
- Copyright holder:
- Dodds et al
- Copyright date:
- 2023
- Rights statement:
- © 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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