MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region.

Journal article

Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single- and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A parallel M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8.

Authors: Jones, E; Xiao, H; Schultz, R; Flaherty, L; Trachtulec, Z

• Publication status: Published
• Journal: Genomics
• Volume: 27
• Issue: 1
• Pages: 40-51
• Publication date: 1995-05-01
• DOI: 10.1006/geno.1995.1006
• EISSN: 1089-8646
• ISSN: 0888-7543
• Language: English
• Keywords: Mice; Molecular Sequence Data; Chromosomes, Artificial, Yeast; Chromosome Mapping; Mice, Inbred C57BL; Genes, MHC Class I; H-2 Antigens; Mice, Inbred C3H; Male; Female; Genetic Markers; Base Sequence; Mice, Inbred BALB C; Animals