In vivo fluorescence imaging of E-selectin: quantitative detection of endothelial activation in a mouse model of arthritis.

Journal article

OBJECTIVE: In vivo optical imaging can delineate at the macroscopic level processes that are occurring at the cellular and molecular levels. E-selectin, a leukocyte adhesion molecule expressed on endothelium, is induced by tumor necrosis factor α (TNFα) and other cytokines involved in the pathogenesis of rheumatoid arthritis (RA). Collagen-induced arthritis (CIA) in mice is widely used to study the disease mechanisms and identify new treatments for RA. The purpose of this study was to demons...

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Authors: Gompels, L; Madden, L; Lim, N; Inglis, J; McConnell, E

• Publication status: Published
• Journal: Arthritis and rheumatism
• Volume: 63
• Issue: 1
• Pages: 107-117
• Publication date: 2011-01-01
• DOI: 10.1002/art.30082
• EISSN: 1529-0131
• ISSN: 0004-3591
• Language: English
• Keywords: Fluorescent Antibody Technique; Cells, Cultured; Tumor Necrosis Factor-alpha; Mice; Dose-Response Relationship, Drug; Endothelium, Vascular; Animals; Cell Line; E-Selectin; Analysis of Variance; Arthritis, Experimental; Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Male