In vitro generation of mature human osteoclasts.

Journal article

Mononuclear precursors of human osteoclasts are found in the CD14(+) monocyte fraction of circulating peripheral blood mononuclear cells (PBMCs). It is possible to generate osteoclasts in vitro from PBMCs cultured with macrophage colony-stimulating factor and receptor activator for nuclear factor κB ligand. In these cultures, however, it is not possible to distinguish the effect of a specific agent on osteoclast resorption activity as opposed to osteoclast differentiation. To produce a popula...

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Authors: Hemingway, F; Cheng, X; Knowles, H; Estrada, F; Gordon, S

• Publication status: Published
• Journal: Calcified tissue international
• Volume: 89
• Issue: 5
• Pages: 389-395
• Publication date: 2011-11-01
• DOI: 10.1007/s00223-011-9530-0
• EISSN: 1432-0827
• ISSN: 0171-967X
• Language: English
• Keywords: Osteoclasts; Humans; Bone Resorption; Diphosphonates; Osteoprotegerin; Imidazoles; Microscopy, Phase-Contrast; Calcitonin; Monocytes; Antigens, CD14