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Journal article

The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.

Abstract:
Cysteines 100, 155, and 197 of recombinant deacetoxycephalosporin C synthase were mutated to alanine residues. The C100A mutant had properties similar to those of the wild-type enzyme, but mutation of Cys-155 and Cys-197 reduced enzyme activity with penicillin N and penicillin G to different extents.
Publication status:
Published

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Publisher copy:
10.1006/bbrc.1999.1957

Authors

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Biochemical and biophysical research communications More from this journal
Volume:
267
Issue:
1
Pages:
445-448
Publication date:
2000-01-01
DOI:
EISSN:
1090-2104
ISSN:
0006-291X
Language:
English
Keywords:
Pubs id:
pubs:31597
UUID:
uuid:ec79c48d-5de7-4003-bdac-94854d9eedb5
Local pid:
pubs:31597
Source identifiers:
31597
Deposit date:
2012-12-19

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