Investigation of non-covalent interactions of amyloidogenic human lysozyme variants and camelid antibodies

Conference item

The investigation of non-covalent interactions of amyloidogenic human lysozyme variants and camelid antibodies was discussed. The two naturally occuring human lysozyme variants were implicated in hereditary systemic amyloidosis. Tri-NAG was shown to bind to both free and associated lysozyme, implying that the enzyme was still active when bound to the antibody. The single-domain camelid antibody appears to bind with its antigen lysozyme and the variants thereof with a 1:1 stoichiometry. Amyloidogenic lysozyme variants undergo a spontaneous cooperative unfolding event in solution which was detectable around pH8.05.

Authors: Caddy, G; Last, A; Hernández, H; Robinson, C

• Host title: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
• Pages: 253-254
• Publication date: 2002-01-01