Journal article icon

Journal article

Crystal structure of Escherichia coli ketopantoate reductase at 1.7 A resolution and insight into the enzyme mechanism.

Abstract:

Ketopantoate reductase (KPR, EC 1.1.1.169) catalyzes the NADPH-dependent reduction of ketopantoate to pantoate on the pantothenate (vitamin B(5)) biosynthetic pathway. The Escherichia coli panE gene encoding KPR was cloned and expressed at high levels as the native and selenomethionine-substituted (SeMet) proteins. Both native and SeMet recombinant proteins were purified by three chromatographic steps, to yield pure proteins. The wild-type enzyme was found to have a K(M)(NADPH) of 20 microM, ...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1021/bi011020w

Authors


Matak-Vinković, D More by this author
Vinković, M More by this author
Saldanha, SA More by this author
Ashurst, JL More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Genomics Consortium
Expand authors...
Journal:
Biochemistry
Volume:
40
Issue:
48
Pages:
14493-14500
Publication date:
2001-12-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:ec09b0af-fba9-4648-bc42-bece6b5174f3
Source identifiers:
32001
Local pid:
pubs:32001

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP