CaM kinase II-dependent mobilization of secretory granules underlies acetylcholine-induced stimulation of exocytosis in mouse pancreatic B-cells.

Journal article

1. Measurements of cell capacitance were used to investigate the mechanisms by which acetylcholine (ACh) stimulates Ca2+-induced exocytosis in single insulin-secreting mouse pancreatic B-cells. 2. ACh (250 microM) increased exocytotic responses elicited by voltage-clamp depolarizations 2.3-fold. This effect was mediated by activation of muscarinic receptors and dependent on elevation of the cytoplasmic Ca2+ concentration ([Ca2+]i) attributable to mobilization of Ca2+ from intracellular stores...

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Authors: Gromada, J; Høy, M; Renström, E; Bokvist, K; Eliasson, L

• Publication status: Published
• Journal: Journal of physiology
• Volume: 518 ( Pt 3)
• Issue: 3
• Pages: 745-759
• Publication date: 1999-08-01
• DOI: 10.1111/j.1469-7793.1999.0745p.x
• EISSN: 1469-7793
• ISSN: 0022-3751
• Language: English
• Keywords: Muscarinic Agonists; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Electrophysiology; Kinetics; Electric Conductivity; Calcium-Calmodulin-Dependent Protein Kinases; Animals; Exocytosis; Membrane Potentials; Acetylcholine; Cytoplasmic Granules; Protein Kinase C; Stimulation, Chemical; Islets of Langerhans; Patch-Clamp Techniques; Calcium; Enzyme Inhibitors; Mice