ED(27) trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype.

Journal article

ED(27) trophoblast-like cells were prepared from human chorionic villus samples obtained at 9 weeks gestation and have been grown continuously in vitro without phenotypic drift for nearly a decade. These cells express many trophoblast markers, including cytokeratin, placental alkaline phosphatase (PLAP), secretion of 17beta-estradiol, and a microvillous apical surface. The ED(27) cell line is a useful model system for studies of placental cell biology and has been distributed to laboratories ...

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Authors: Kniss, D; Xie, Y; Li, Y; Kumar, S; Linton, E

• Publication status: Published
• Journal: Placenta
• Volume: 23
• Issue: 1
• Pages: 32-43
• Publication date: 2002-01-01
• DOI: 10.1053/plac.2001.0749
• EISSN: 1532-3102
• ISSN: 0143-4004
• Language: English
• Keywords: Histocompatibility Antigens Class I; Hela Cells; Humans; Pregnancy Trimester, First; Polymerase Chain Reaction; Adult; Equipment Contamination; Female; Chorionic Villi; Cell Line, Transformed; Trophoblasts; Haplotypes; Biological Markers; DNA; Phenotype; Culture Techniques; Pregnancy; Flow Cytometry; Immunohistochemistry