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Journal article

Gentle, fast and effective crystal soaking by acoustic dispensing.

Abstract:

The steady expansion in the capacity of modern beamlines for high-throughput data collection, enabled by increasing X-ray brightness, capacity of robotics and detector speeds, has pushed the bottleneck upstream towards sample preparation. Even in ligand-binding studies using crystal soaking, the experiment best able to exploit beamline capacity, a primary limitation is the need for gentle and nontrivial soaking regimens such as stepwise concentration increases, even for robust and well charac...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1107/s205979831700331x

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Genomics Consortium
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Genomics Consortium
Role:
Author
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Bayer Pharma AG More from this funder
Canada Foundation for Innovation More from this funder
Boehringer Ingelheim More from this funder
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Publisher:
International Union of Crystallography Publisher's website
Journal:
Acta Crystallographica Section D: Biological Crystallography Journal website
Volume:
73
Pages:
246-255
Publication date:
2017-03-01
Acceptance date:
2017-02-28
DOI:
ISSN:
2059-7983
Pmid:
28291760
Source identifiers:
686907
Language:
English
Keywords:
Pubs id:
pubs:686907
UUID:
uuid:ebc283ae-896a-4319-9bc6-7d7b4346a681
Local pid:
pubs:686907
Deposit date:
2017-08-21

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