Human CD68 promoter GFP transgenic mice allow analysis of monocyte to macrophage differentiation in vivo.

Journal article

The recruitment of monocytes and their differentiation into macrophages at sites of inflammation are key events in determining the outcome of the inflammatory response and initiating the return to tissue homeostasis. To study monocyte trafficking and macrophage differentiation in vivo, we have generated a novel transgenic reporter mouse expressing a green fluorescent protein (GFP) under the control of the human CD68 promoter. CD68-GFP mice express high levels of GFP in both monocyte and embry...

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Authors: Iqbal, A; McNeill, E; Kapellos, T; Regan-Komito, D; Norman, S

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: American Society of Hematology
• Journal: Blood
• Volume: 124
• Issue: 15
• Pages: e33-e44
• Publication date: 2014-10-01
• DOI: 10.1182/blood-2014-04-568691
• EISSN: 1528-0020
• ISSN: 0006-4971
• Language: English
• Keywords: Mycobacterium bovis; Humans; Receptors, Chemokine; Bone Marrow; Promoter Regions, Genetic; Spleen; Macrophages, Peritoneal; Mycobacterium Infections; Flow Cytometry; Chronic Disease; Embryonic Development; Mice, Inbred C57BL; Antigens, Differentiation, Myelomonocytic; Green Fluorescent Proteins; Animals; Cell Differentiation; Monocytes; Fluorescent Antibody Technique; Inflammation; Genes, Reporter; Adoptive Transfer; Phenotype; Leukocytes; Antigens, CD; Mice, Transgenic