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Journal article

Low-cost, frequency-domain, fluorescence lifetime confocal microscopy.

Abstract:

We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime imag...

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Publication status:
Published

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Publisher copy:
10.1111/j.0022-2720.2004.01316.x

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
Journal:
Journal of microscopy More from this journal
Volume:
214
Issue:
Pt 1
Pages:
36-42
Publication date:
2004-04-01
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
Language:
English
Keywords:
Pubs id:
pubs:62452
UUID:
uuid:eb79270f-217d-4bd7-9672-3753e21de11f
Local pid:
pubs:62452
Source identifiers:
62452
Deposit date:
2013-11-17

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