Journal article

Low-cost, frequency-domain, fluorescence lifetime confocal microscopy.

Abstract:: We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime images of biological specimens from a single confocal scan.

Publication status:: Published

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APA Style

Booth, M., & Wilson, T. (2004). Low-cost, frequency-domain, fluorescence lifetime confocal microscopy. Journal of Microscopy, 214(Pt 1), 36–42.

MLA Style

Booth, M, and T Wilson. “Low-Cost, Frequency-Domain, Fluorescence Lifetime Confocal Microscopy.” Journal of Microscopy, vol. 214, no. Pt 1, 2004, pp. 36–42.

Chicago Style

Booth, M, and T Wilson. 2004. “Low-Cost, Frequency-Domain, Fluorescence Lifetime Confocal Microscopy.” Journal of Microscopy 214 (Pt 1): 36–42.
Print

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Publisher copy:: 10.1111/j.0022-2720.2004.01316.x

Authors

+ Booth, M More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Engineering Science
Role:: Author

+ Wilson, T More by this author

Role:: Author

Journal:: Journal of microscopy More from this journal
Volume:: 214
Issue:: Pt 1
Pages:: 36-42
Publication date:: 2004-04-01
DOI:: 10.1111/j.0022-2720.2004.01316.x
EISSN:: 1365-2818
ISSN:: 0022-2720

Language:: English
Keywords:: Electronics

Image Processing, Computer-Assisted

Microscopy, Fluorescence

Microscopy, Confocal

Tobacco
Pubs id:: pubs:62452
UUID:: uuid:eb79270f-217d-4bd7-9672-3753e21de11f
Local pid:: pubs:62452
Source identifiers:: 62452
Deposit date:: 2013-11-17
ARK identifier:: ark:/29072/ora_eb79270f217d4bd796723753e21de11f

Terms of use

Copyright date:: 2004

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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