Journal article
Low-cost, frequency-domain, fluorescence lifetime confocal microscopy.
- Abstract:
-
We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime imag...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Journal of microscopy More from this journal
- Volume:
- 214
- Issue:
- Pt 1
- Pages:
- 36-42
- Publication date:
- 2004-04-01
- DOI:
- EISSN:
-
1365-2818
- ISSN:
-
0022-2720
Item Description
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:62452
- UUID:
-
uuid:eb79270f-217d-4bd7-9672-3753e21de11f
- Local pid:
-
pubs:62452
- Source identifiers:
-
62452
- Deposit date:
-
2013-11-17
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- Copyright date:
- 2004
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