Tracking antigen-experienced effector T cells in vitro and in vivo.

Journal article

The TCR complex is a multisubunit complex, comprising at least eight transmembrane units. The clonotypic TCR alpha and beta chains are responsible for antigen recognition, whilst the invariant chains of the CD3 complex (delta, epsilon and gamma) and two zeta (zeta) polypeptides couple antigen recognition to downstream signal transduction pathways. TCRzeta (CD247) functions as an amplification module in the TCR signalling cascade and is also essential for the assembly and surface expression of...

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Authors: Gorman, C; Monaco, C; Ammiratti, E; Vermi, A; Marelli-Berg, F

• Journal: Methods in molecular biology (Clifton, N.J.)
• Volume: 616
• Pages: 253-267
• Publication date: 2010-01-01
• DOI: 10.1007/978-1-60761-461-6_16
• EISSN: 1940-6029
• ISSN: 1064-3745
• Language: English
• Keywords: Intracellular Space; Blood Donors; Biological Assay; Flow Cytometry; Cells, Cultured; Antigens; T-Lymphocytes; Staining and Labeling; Endothelial Cells; Leukocytes, Mononuclear; Humans; Centrifugation, Density Gradient; Receptors, Antigen, T-Cell; Cell Separation; Cell Movement