Drosophila melanogaster SL2 cells contain a hypoxically inducible DNA binding complex which recognises mammalian HIF-binding sites.

Journal article

Nuclear extracts from Drosophila SL2 cells were found to contain a hypoxically inducible complex capable of binding to hypoxia response elements from mammalian genes. This complex (HIF-D) resembled mammalian hypoxia inducible factor (HIF-1) in DNA sequence specificity, abrogation of induction by cycloheximide, induction by desferrioxamine and redox sensitivity of DNA binding. However, HIF-D was not induced by cobalt and was less sensitive to phosphatase than HIF-1. Endogenous phosphoglycerate kinase mRNA in SL2 cells showed similar inducible characteristics to HIF-D. These findings are evidence that the mammalian HIF-1 dependent system of oxygen regulated gene expression has a functional homologue in Drosophila.

Authors: Nagao, M; Ebert, B; Ratcliffe, P; Pugh, C

• Publication status: Published
• Journal: FEBS letters
• Volume: 387
• Issue: 2-3
• Pages: 161-166
• Publication date: 1996-06-01
• DOI: 10.1016/0014-5793(96)00484-x
• EISSN: 1873-3468
• ISSN: 0014-5793
• Language: English
• Keywords: Animals; Nuclear Proteins; Cell Hypoxia; DNA-Binding Proteins; DNA Probes; Molecular Sequence Data; Humans; Phosphoric Monoester Hydrolases; Dithiothreitol; Cobalt; Drosophila melanogaster; Cycloheximide; Binding Sites; Hypoxia-Inducible Factor 1; Transcription Factors; Hypoxia-Inducible Factor 1, alpha Subunit; Deferoxamine; Phosphoglycerate Kinase; Base Sequence; Cell Line; Hela Cells; Mice