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Validation of single real-time TaqMan PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens.

Abstract:

Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt 5207-5292) for the detection and quantification of the four major genotypes of HEV. This assay proved to be as efficient as the conventional RT-PCR methodology for the detection of HEV in clinical...

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Publication status:
Published

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Publisher copy:
10.1002/jmv.20665

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Journal:
Journal of medical virology
Volume:
78
Issue:
8
Pages:
1076-1082
Publication date:
2006-08-05
DOI:
EISSN:
1096-9071
ISSN:
0146-6615
URN:
uuid:eb29ee52-545a-486e-8407-ac10c8c771a2
Source identifiers:
68247
Local pid:
pubs:68247

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