Evidence for macromolecular protein rings in the absence of bulk water.
We have examined the architecture of a protein complex in the absence of bulk water. By determining collision cross sections of assemblies of the trp RNA binding protein, TRAP, we established that the 11-membered ring topology of the complex can be maintained within a mass spectrometer. We also found that the binding of tryptophan enhances the stability of the ring structure and that addition of a specific RNA molecule increases the size of the complex and prevents structural collapse. These ...Expand abstract
- Publisher copy:
- Copyright date:
- PubMed ID: 16293722