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Visualizing cytoophidia expression in drosophila follicle cells via immunohistochemistry

Abstract:: We describe a user-friendly immunohistochemical approach for the detection of protein localization in Drosophila ovaries, here focusing on CTP synthase. This approach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond Drosophila ovaries.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Yilmaz-Tastan, O., & Liu, J. (2015). Visualizing cytoophidia expression in drosophila follicle cells via immunohistochemistry. In Methods in Molecular Biology (Vol. 1328, pp. 179–189). Springer.

MLA Style

Yilmaz-Tastan, O, and J Liu. “Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry.” Methods in Molecular Biology, vol. 1328, Springer, 2015, pp. 179–89.

Chicago Style

Yilmaz-Tastan, O, and J Liu. 2015. “Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry.” In Methods in Molecular Biology, vol. 1328. Springer.
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Files:: 318711_1_En_13.pdf

(Preview, Accepted manuscript, pdf, 513.7KB, Terms of use)

Publisher copy:: 10.1007/978-1-4939-2851-4_13

Authors

+ Yilmaz-Tastan, O More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Physiology Anatomy & Genetics
Role:: Author

+ Liu, J More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Physiology Anatomy & Genetics
Role:: Author

+ Medical Research Council More from this funder

Publisher:: Springer
Host title:: Methods in Molecular Biology
Volume:: 1328
Pages:: 179-189
Publication date:: 2015-09-01
DOI:: 10.1007/978-1-4939-2851-4_13
EISSN:: 1940-6029
ISSN:: 1064-3745

Keywords:: Ovary

Cytoophidium

Immunohistochemistry

Follicle cell

Antigen

Drosophila

Antibody
Pubs id:: pubs:567566
UUID:: uuid:ea3f7bc1-0939-474b-840c-f0bc2384ed2f
Local pid:: pubs:567566
Source identifiers:: 567566
Deposit date:: 2016-04-04
ARK identifier:: ark:/29072/ora_ea3f7bc10939474b840cf0bc2384ed2f

Terms of use

Copyright holder:: Springer
Copyright date:: 2015
Notes:: © Springer Science+Business Media New York 2015. This is the accepted manuscript version of the article. The final version is available online from Springer at: [10.1007/978-1-4939-2851-4_13]

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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