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Electrophilic affibodies forming covalent bonds to protein targets.

Abstract:

Antibody affinity limits sensitivity of detection in many areas of biology and medicine. High affinity usually depends on achieving the optimal combination of the natural 20 amino acids in the antibody binding site. Here, we investigate the effect on recognition of protein targets of placing an unnatural electrophile adjacent to the target binding site. We positioned a weak electrophile, acrylamide, near the binding site between an affibody, a non-immunoglobulin binding scaffold, and its prot...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m109.034322

Authors


Howarth, M More by this author
Journal:
The Journal of biological chemistry
Volume:
284
Issue:
47
Pages:
32906-32913
Publication date:
2009-11-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:e9d51fe0-a014-431b-a5fd-42392c046f57
Source identifiers:
101211
Local pid:
pubs:101211

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