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Analysis of membrane-localized binding kinetics with FRAP.

Abstract:

Interactions between plasma membrane-associated proteins on interacting cells are critical for many important biological processes. Few experimental techniques, however, can accurately determine the association and the dissociation rates between such interacting pairs when the two molecules diffuse on apposing membranes or lipid bilayers. In this study, we give a theoretical description of how and when fluorescence recovery after photobleaching (FRAP) experiments can be used to quantify these...

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Publisher copy:
10.1007/s00249-008-0286-z

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
European biophysics journal : EBJ
Volume:
37
Issue:
5
Pages:
627-638
Publication date:
2008-06-05
DOI:
EISSN:
1432-1017
ISSN:
0175-7571
URN:
uuid:e999d537-22ab-4233-b6bc-f946d8e3bd1c
Source identifiers:
237087
Local pid:
pubs:237087

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