Journal article
Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins
- Abstract:
- Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
-
-
(Preview, Accepted manuscript, pdf, 409.4KB, Terms of use)
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- Publisher copy:
- 10.1038/s41592-020-0821-0
Authors
- Publisher:
- Springer Nature
- Journal:
- Nature Methods More from this journal
- Volume:
- 17
- Issue:
- 5
- Pages:
- 505-508
- Publication date:
- 2020-05-04
- Acceptance date:
- 2020-03-25
- DOI:
- EISSN:
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1548-7105
- ISSN:
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1548-7091
- Pmid:
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32371966
- Language:
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English
- Pubs id:
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1103434
- Local pid:
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pubs:1103434
- Deposit date:
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2020-07-16
- ARK identifier:
Terms of use
- Copyright holder:
- Gault et al.
- Copyright date:
- 2020
- Rights statement:
- © The Author(s), under exclusive licence to Springer Nature America, Inc. 2020
- Notes:
- This is the accepted manuscript version of the article. The final version is available from Springer Nature at: https://doi.org/10.1038/s41592-020-0821-0
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