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Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins

Abstract:
Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41592-020-0821-0

Authors


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Division:
MSD
Sub department:
AL BIOCHEMISTRY; DN PHYSICAL & THEORETICAL CHEM
Oxford college:
Queen's College
Role:
Author
ORCID:
0000-0001-7686-2983
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Role:
Author
ORCID:
0000-0002-7958-4074
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Department:
CHEMISTRY
Sub department:
Physical & Theoretical Chem
Role:
Author
ORCID:
0000-0003-4346-182X
Publisher:
Springer Nature
Journal:
Nature Methods More from this journal
Volume:
17
Issue:
5
Pages:
505-508
Publication date:
2020-05-04
Acceptance date:
2020-03-25
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pmid:
32371966
Language:
English
Pubs id:
1103434
Local pid:
pubs:1103434
Deposit date:
2020-07-16

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