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Ultra-fast tandem mass spectrometry scanning combined with monolithic column liquid chromatography increases throughput in proteomic analysis.

Abstract:

Liquid chromatography combined with electrospray ionization mass spectrometry (LC/ESI-MS) has been used successfully for the characterization of biomolecules in proteomics in the last few years. This methodology relied largely on the use of reversed-phase chromatography, in particular C18-based resins, which are suitable for separation of peptides. Here we show that polymeric [polystyrene divinylbenzene] monolithic columns can be used to separate peptide mixtures faster and at a higher resolu...

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Publication status:
Published

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Publisher copy:
10.1002/rcm.2563

Authors


Batycka, M More by this author
Inglis, NF More by this author
Fraser-Pitt, D More by this author
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Journal:
Rapid communications in mass spectrometry : RCM
Volume:
20
Issue:
14
Pages:
2074-2080
Publication date:
2006
DOI:
EISSN:
1097-0231
ISSN:
0951-4198
URN:
uuid:e95996b8-e88c-4362-888d-4bfd1c9f5367
Source identifiers:
6763
Local pid:
pubs:6763

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