Evaluation of the Standard Diagnostics Leptospira IgM ELISA for diagnosis of acute leptospirosis in Lao PDR

Journal article

The diagnostic utility of the Standard Diagnostics . Leptospira IgM ELISA for detection of acute leptospirosis was assessed in febrile adults admitted in Vientiane, Laos. Using the cut-off suggested by the manufacturer [optical density (OD) ≥0.75], the assay demonstrated limited diagnostic capacity with a sensitivity of 95% and a specificity of 41% compared with the . Leptospira microscopic agglutination test, which is the serological gold standard. However, re-evaluation of the diagnostic cut-off to an OD of 1.7 demonstrated improved diagnostic accuracy overall (sensitivity 70%; specificity 78%). © 2012 Royal Society of Tropical Medicine and Hygiene.

Authors: Tanganuchitcharnchai, A; Smythe, L; Dohnt, M; Hartskeerl, R; Vongsouvath, M

• Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene
• Volume: 106
• Issue: 9
• Pages: 563-566
• Publication date: 2012-09-01
• DOI: 10.1016/j.trstmh.2012.06.002
• EISSN: 1878-3503
• ISSN: 0035-9203
• Language: English
• Keywords: Cut-off; Diagnosis; Leptospira; ELISA