Translesion synthesis: Y-family polymerases and the polymerase switch.

Journal article

Replicative DNA polymerases are blocked at DNA lesions. Synthesis past DNA damage requires the replacement of the replicative polymerase by one of a group of specialised translesion synthesis (TLS) polymerases, most of which belong to the Y-family. Each of these has different substrate specificities for different types of damage. In eukaryotes mono-ubiquitination of PCNA plays a crucial role in the switch from replicative to TLS polymerases at stalled forks. All the Y-family polymerases have ubiquitin binding sites that increase their binding affinity for ubiquitinated PCNA at the sites of stalled forks.

Authors: Lehmann, A; Niimi, A; Ogi, T; Brown, S; Sabbioneda, S

• Publication status: Published
• Journal: DNA repair
• Volume: 6
• Issue: 7
• Pages: 891-899
• Publication date: 2007-07-01
• DOI: 10.1016/j.dnarep.2007.02.003
• EISSN: 1568-7856
• ISSN: 1568-7864
• Language: English
• Keywords: DNA Repair; Animals; DNA-Directed DNA Polymerase; Models, Biological; DNA Replication; Ubiquitin; Proliferating Cell Nuclear Antigen; Humans