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A comparative study of digoxigenin, 2,4-dinitrophenyl, and alkaline phosphatase as deoxyoligonucleotide labels in non-radioisotopic in situ hybridisation.

Abstract:

AIM: To determine the optimum form of labelling and the most efficient reporter molecule for non-radioisotopic in situ hybridisation (ISH). METHODS: Nine deoxyoligonucleotides complementary to histone mRNA were synthesised and labelled either enzymatically or during solid-phase synthesis with the reporter molecules digoxigenin, 2,4-dinitrophenyl (DNP), or alkaline phosphatase. Pooled deoxyoligonucleotide cocktails were then used in non-radioisotopic ISH detection of histone mRNA in human tons...

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Publication status:
Published

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Publisher copy:
10.1136/jcp.50.8.686

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Journal:
Journal of clinical pathology More from this journal
Volume:
50
Issue:
8
Pages:
686-690
Publication date:
1997-08-01
DOI:
EISSN:
1472-4146
ISSN:
0021-9746
Language:
English
Keywords:
Pubs id:
pubs:400277
UUID:
uuid:e8ec4fd3-91b2-46f4-8170-a874c6af9e44
Local pid:
pubs:400277
Source identifiers:
400277
Deposit date:
2013-11-16

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