Cloning and nucleotide sequence analysis of a gene encoding an OXA-derived beta-lactamase in Acinetobacter baumannii.

Journal article

A clinical strain of Acinetobacter baumannii (strain Ab41) that was resistant to all beta-lactam antibiotics tested except ceftazidime, ceftriaxone, ceftizoxime, and imipenem produced three beta-lactamases: a presumptive chromosomal cephalosporinase, a TEM-1-like beta-lactamase (pI 5.4), and a novel OXA-derived beta-lactamase named OXA-21 (pI 7.0). The gene encoding OXA-21 was located in an integron. The nucleotide sequence showed three mutations compared with the sequence of OXA-3, with two being silent; the nonsilent mutation generated a substitution of Ile-217 to Met.

Authors: Vila, J; Navia, M; Ruiz, J; Casals, C

• Publication status: Published
• Journal: Antimicrobial agents and chemotherapy
• Volume: 41
• Issue: 12
• Pages: 2757-2759
• Publication date: 1997-12-01
• EISSN: 1098-6596
• ISSN: 0066-4804
• Language: English
• Keywords: Acinetobacter; Isoelectric Focusing; Cloning, Molecular; Amino Acid Sequence; Molecular Sequence Data; Base Sequence; beta-Lactamases; Sequence Analysis, DNA