p53 expression in normal paraffin-embedded tissue using different antibodies and antigen retrieval buffer systems.

Journal article

AIMS: The study was undertaken to demonstrate wild-type p53 in normal paraffin-embedded tissues using two widely used antibodies, DO7 and 1801 and two different antigen retrieval buffer systems. METHODS AND RESULTS: Formalin-fixed paraffin-embedded normal tissue samples were obtained from the archives of the John Radcliffe Hospital, Oxford. Antigen retrieval was performed by microwaving using two different buffer systems: (i) the commercially available Dako target retrieval solution (Cat. no....

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Authors: Pillai, G; Roberts, H; Gatter, K; Pezzella, F

• Publication status: Published
• Journal: Histopathology
• Volume: 42
• Issue: 1
• Pages: 83-87
• Publication date: 2003-01-01
• DOI: 10.1046/j.1365-2559.2003.01563.x
• EISSN: 1365-2559
• ISSN: 0309-0167
• Language: English
• Keywords: Immunoenzyme Techniques; Epitopes; Buffers; Antigens; Staining and Labeling; Tumor Suppressor Protein p53; Humans; Female; Paraffin Embedding; Male; Reagent Kits, Diagnostic