Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes.

Journal article

Modification of proteins with ubiquitin (Ub) and Ub-like modifiers (Ubls) plays a fundamental role in cell biology. As a consequence, proteomics-based efforts were developed to characterize proteins that are modified by Ub or Ubls. A more focused functional proteomics strategy relies on active-site probes based on the Ub/Ubl scaffold, which specifically targets Ub/Ubl-processing enzymes. Activity-based profiling with such tools led to the identification of novel gene products with Ub/Ubl-processing activity and uncovered novel control mechanisms regulating their activity. This review discusses recent advances in chemistry-based functional proteomics applications, and how this information can provide a framework for drug development against Ub/Ubl-processing enzymes.

Authors: Kessler, B

• Publication status: Published
• Journal: Expert review of proteomics
• Volume: 3
• Issue: 2
• Pages: 213-221
• Publication date: 2006-04-01
• DOI: 10.1586/14789450.3.2.213
• EISSN: 1744-8387
• ISSN: 1478-9450
• Language: English
• Keywords: Proteomics; Catalytic Domain; Substrate Specificity; Animals; Ubiquitin-Protein Ligase Complexes; Protein Binding; Humans; Ubiquitins