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The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation.

Abstract:

BACKGROUND: The isolation of islets from the human pancreas critically depends on an efficient enzyme blend. Previous studies have solely focused on the presence of collagenase and neutral protease/thermolysin. Despite improved characterization of these components, the lot-related variability in efficacy still persists suggesting that additional so far disregarded enzymes are required for efficient islet cleavage. METHODS: Varying activities of a tryptic-like enzyme were identified within co...

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Publisher copy:
10.1097/tp.0b013e31819499f0

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Institution:
University of Oxford
Department:
Oxford, MSD, Surgical Sciences
Role:
Author
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Journal:
Transplantation
Volume:
87
Issue:
3
Pages:
370-375
Publication date:
2009-02-05
DOI:
EISSN:
1534-6080
ISSN:
0041-1337
URN:
uuid:e7b498d3-8ed3-4214-a7c6-60ce2b31dc93
Source identifiers:
269565
Local pid:
pubs:269565

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